Current address: ucla school of dentistry...

Cell,,

Pages 143-156,

Published December 23, 2010

© 2011 Elsevier Inc. All rights reserved. DOI: 10. 1016 / in. cage. In 2010. 11. 052

Robert G. Nichols

Christine M. Kazmierczak

Karis J. Lee

Malcolm D. Winkler

Neva J. Krogan

Carol A. Gross

Oral and Craniofacial Sciences Higher program, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA

Department of Microbiology and Immunology, University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA

Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, California 94107, USA

Department of Cell and Molecular Pharmacology and California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, California 94158, USA

Faculty of Biology, Indiana University, Bloomington 47405, USA

Department of Biology and Rosenstiel basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 -9110, USA

Department of Cell and Tissue Biology, University of California at San Francisco, San Francisco, California 94158, USA

Current address: UCLA School of Dentistry, 10833 Le Conte Avenue, Los Angeles, CA 90095 -1762, USA

Present address: Massachusetts General Hospital, 50 Staniford Street, Boston, MA 02114, USA

► Phenomic profiling of Escherichia coli

explosion of sequence information in cheap strattera bacteria makes the development of high performance, cost-effective approaches according to the genes to phenotypes. Use

E. coli as a proof of principle, we show that combining large-scale chemical genomics quantitative measurement of fitness provides high quality data set rich in discoveries. Probing growth account mutant library of hundreds of conditions in parallel gave 10 000 phenotypes that allowed us to study gene essentiality, open leads to the function of genes and the drug, and to understand higher-order organization of the bacterial chromosome. We publish new information derived from research, including understanding the genes involved in resistance to various antibiotics and interaction between widely used combinatorial therapy with antibiotics, trimethoprim and sulfonamides. This set of data publicly available on http://ecoliwiki. net / tools / chemgen /, is a valuable resource for the microbiological and bioinformatics communities, as it provides high confidence in the relationship between hundreds and annotated genes neoharakterizovannoy and conclusions about how the actions of several poorly understood drug. .